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Related Experiment Video

Updated: Jun 5, 2026

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

Molecular phylogeny of eukaryotes.

¹Martin Schlegel is at the Universität Tübingen, Zoologisches Institut, Abteilung Zellbiologie, Auf der Morgenstelle 28, D-72076 Tübingen, Germany.

Trends in Ecology & Evolution

|January 18, 2011

Summary

Phylogenetic analyses using ribosomal RNA and protein-coding genes reveal new insights into eukaryote evolution. These studies suggest archaebacteria and eukaryotes are closely related, with unique branching patterns in protists.

Area of Science:

Molecular Biology
Evolutionary Biology
Phylogenetics

Background:

Traditional classifications relied on morphology.
Ribosomal RNAs (rRNAs) and protein-coding genes offer deeper evolutionary insights.
Eukaryote phylogeny remains a complex area of study.

Purpose of the Study:

To reconstruct the evolutionary history of eukaryotes.
To investigate the relationship between archaebacteria and eukaryotes.
To understand the early diversification of eukaryotes and the origin of mitochondria.

Main Methods:

Comparative analysis of ribosomal RNA sequences.
Analysis of paralogous protein-coding genes.
Phylogenetic tree construction.

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A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

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Last Updated: Jun 5, 2026

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

Main Results:

Archaebacteria and eukaryotes are identified as sister groups based on gene analyses.
Small subunit rRNA diversity in protists is exceptionally high.
A distinct lineage of mitochondrion-lacking taxa emerges early in the rRNA tree.
Re-evaluation of ancestral relationships for several eukaryotic clades.
Sequence comparisons enable tracing of secondary endosymbiotic events.

Conclusions:

Ribosomal RNA and protein-coding gene comparisons provide a robust framework for eukaryote phylogeny.
The early branching of mitochondrion-lacking eukaryotes challenges previous assumptions.
Molecular data allows for detailed reconstruction of endosymbiotic events in eukaryote evolution.