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Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

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Introduction to Solid Supported Membrane Based Electrophysiology
19:56

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Published on: May 11, 2013

Development of polymer-membrane based electrodes for suramin.

Andrew Yu1, Brandon Shepherd, Meghan Wagner

  • 1Department of Chemistry and Biochemistry, Otterbein University, Westerville, OH 43081, USA.

Analytica Chimica Acta
|January 18, 2011
PubMed
Summary

A new polymer membrane electrode accurately measures the anionic drug suramin in biological fluids. Optimized components allow adjustable dynamic range for precise suramin quantification, even in human plasma.

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Area of Science:

  • Electrochemistry
  • Materials Science
  • Analytical Chemistry

Background:

  • Suramin is an anionic drug with therapeutic and toxic ranges.
  • Accurate measurement of suramin in biological samples is crucial for patient management.
  • Existing methods may be affected by background anionic concentrations.

Purpose of the Study:

  • To develop a polymer membrane-based electrode for suramin quantification.
  • To optimize the electrode's membrane composition for a specific dynamic range.
  • To evaluate the electrode's performance in biological samples and compare with titration methods.

Main Methods:

  • Fabrication of polymer membrane electrodes using specific compositions.
  • Measurement of electromotive force (EMF) response to suramin in buffered saline and biological samples.
  • Potentiometric titration with polycationic protamine monitored by a protamine-sensitive electrode.

Main Results:

  • A large non-equilibrium, steady-state EMF response to suramin was observed.
  • An optimized membrane composition (25% tridodecylmethyl ammonium chloride, 55% bis-2-ethylhexyl sebacate, 20% Pellethane) was determined.
  • The electrode successfully quantified suramin in human plasma, with titration offering improved accuracy against background anions.

Conclusions:

  • A novel polymer membrane electrode provides a viable method for suramin measurement.
  • Electrode performance, including dynamic range, can be tuned by membrane composition.
  • Potentiometric titration with protamine offers a robust alternative for accurate suramin determination in complex biological matrices.