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Updated: Jun 5, 2026

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Published on: March 17, 2015
Corticotropin-releasing hormone inhibits in vitro oocyte maturation in mice
Erasmia Kiapekou1, Evangelia Zapanti, Dionysia Voukelatou
11st Department of Obstetrics and Gynecology, Alexandra Hospital, University of Athens, Athens, Greece. ekiapek@otenet.gr
Abstract:
The expression of corticotropin-releasing hormone (CRH) receptor 1 messenger RNA in stages of follicle growth was examined by reverse transcriptase-polymerase chain reaction in long-term cultures of early preantral mouse follicles with and without CRH addition. Corticotropin-releasing hormone receptor 1 is present in stages of mouse follicle growth, whereas 10(-9), 10(-7), and 10(-6) mol/L CRH inhibits oocyte maturation in vitro, an effect reversed by antalarmin addition.
