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Related Experiment Videos

Enzyme immunoassay for flecainide.

J P Freche1, G Coudert, M Wellman

  • 1Centre du Medicament, URA CNRS no. 597, Nancy, France.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|October 15, 1990
PubMed
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Researchers developed a highly specific enzyme immunoassay (ELISA) for measuring plasma flecainide. This new method offers accurate quantification of flecainide, crucial for therapeutic drug monitoring.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Immunology

Background:

  • Accurate quantification of flecainide is essential for effective therapeutic drug monitoring.
  • Existing methods may lack the specificity or sensitivity required for precise plasma flecainide level determination.

Purpose of the Study:

  • To develop and validate a highly specific enzyme immunoassay (ELISA) for the quantitative measurement of plasma flecainide levels.

Main Methods:

  • Synthesis of flecainide-hemisuccinate-BSA conjugate as an immunogen.
  • Raising a specific antiserum in rabbits.
  • Preparation of enzyme-labeled flecainide using horse radish peroxidase via the mixed anhydride method.

Main Results:

  • The developed ELISA demonstrated a detection limit of 10 ng/ml and linearity up to 1,000 µg/l.

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  • Intra-assay and inter-assay variations were low, ranging from 5.1% to 7.8%.
  • Minimal cross-reactivity (<0.1%) was observed with flecainide metabolites and related drugs, while structural analogues showed up to 25% cross-reactivity.

    • Conclusions:

    • The developed ELISA is a sensitive and specific method for measuring plasma flecainide.
    • The study highlights the significance of the aromatic ring structure in the drug's immunogenic properties.
    • This assay is suitable for clinical applications requiring precise flecainide quantification.