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Published on: June 29, 2021
Synthesis of cross-reactive carbohydrate determinants fragments as tools for in vitro allergy diagnosis
Mayeul Collot1, Iain B H Wilson, Merima Bublin
1Ecole Normale Supérieure, Département de Chimie, Université Paris 6, UMR CNRS 7203, 24 rue Lhomond, 75005 Paris, France.
Abstract:
Four biotinylated tri and tetrasaccharide fragments of plant and invertebrate N-glycans were synthesized using methyl tert-butyl phenyl (MBP) thioglycosides donors in order to evaluate their involvement in cross-allergies as cross-reactive carbohydrate determinants (CCDs). Various levels of reactivity to anti-bee and anti-HRP antibodies and with sera from allergic patients were observed when the conjugates were coated on streptavidin microplates. The results showed the potential utility of these xylosylated and fucosylated oligosaccharide fragments in determining CCD antibody epitopes.
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