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Related Concept Videos

Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
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Radical Autoxidation01:20

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Lipid Catabolism01:25

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Overview of Fatty Acid Metabolism01:28

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Updated: Jun 5, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Advanced lipoxidation end-products.

Reinald Pamplona1

  • 1Department of Experimental Medicine, Faculty of Medicine, University of Lleida-IRBLleida, c/Montserrat Roig-2, E-25008 Lleida, Spain. reinald.pamplona@mex.udl.cat

Chemico-Biological Interactions
|January 18, 2011
PubMed
Summary
This summary is machine-generated.

Reactive carbonyl species (RCS) cause molecular damage, forming advanced lipoxidation end-products (ALEs) that contribute to aging and cellular dysfunction. These compounds can spread, leading to widespread tissue damage and loss of biomolecular function.

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Last Updated: Jun 5, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Aging

Background:

  • Reactive carbonyl species (RCS) arise from lipid peroxidation.
  • RCS can modify cellular components nonenzymatically.
  • These modifications are implicated in aging and cellular damage.

Purpose of the Study:

  • To elucidate the role of RCS in cellular damage and aging.
  • To understand the formation and impact of advanced lipoxidation end-products (ALEs).

Main Methods:

  • Analysis of chemical modifications induced by RCS.
  • Investigation of the diffusion and reactivity of RCS.
  • Assessment of downstream effects on biomolecules and cellular function.

Main Results:

  • RCS formation leads to advanced lipoxidation end-products (ALEs).
  • RCS are stable and can diffuse, causing damage distant from their origin.
  • Modification of biomolecules by RCS results in loss of function and structural integrity.

Conclusions:

  • RCS-induced modifications contribute significantly to the aging process.
  • The cytotoxic effects of RCS are mediated by ALE formation.
  • Cellular dysfunction and tissue damage are consequences of widespread biomolecular modification by RCS.