Related Experiment Video
Updated: Jun 5, 2026
Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41
Published on: March 25, 2010
Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme
Guoqing Zhang1, Qing Cao, Na Li
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Abstract:
A novel affinity purification method for lysozyme (LZM) based on functionalized magnetic microspheres was developed. Tris(hydroxymethyl)aminomethane (Tris)-modified magnetic microspheres with specific affinity toward LZM were prepared using Tris as ligand and silica-coated magnetic microshperes as support. Transmission electron microscopy and magnetic property measurement results showed that the Tris-modified magnetic microspheres have a very good core-shell structure and high magnetization.The maximum binding capacity of LZM was about 108.6 mg/g magnetic microspheres. LZM purified from chicken egg white had high purity and well-maintained activity of 8140 U/mg. This magnetic-mediated LZM purification strategy has advantages of high efficiency, low cost and easy operation.
More Related Videos
10:21Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
Published on: January 13, 2023
07:33Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
Published on: April 26, 2011