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Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

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Updated: Jun 5, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Ultrafast optical multidimensional spectroscopy without interferometry.

J A Davis1, T R Calhoun, K A Nugent

  • 1ARC Centre of Excellence for Coherent X-Ray Science, The School of Physics, The University of Melbourne, Victoria 3010, Australia. JDavis@swin.edu.au

The Journal of Chemical Physics
|January 19, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel phase retrieval technique for multidimensional optical spectroscopy, enabling access to previously unavailable modalities. The iterative algorithm accurately recovers phase information, advancing chemical and biological analysis.

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Last Updated: Jun 5, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Published on: December 30, 2025

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

Area of Science:

  • Optical Spectroscopy
  • Chemical Physics
  • Biophysics

Background:

  • Multidimensional optical spectroscopy offers insights into electronic processes.
  • Current techniques are limited by interferometric detection requirements.
  • Existing methods are analogous to multidimensional NMR spectroscopies.

Purpose of the Study:

  • To present a phase retrieval technique for accessing new multidimensional optical spectroscopy modalities.
  • To detail an iterative algorithm for recovering phase relationships in two-color spectroscopy.
  • To provide practical implementation guidance for phase retrieval algorithms.

Main Methods:

  • Development of an iterative algorithm for phase retrieval.
  • Application to two-color multidimensional spectroscopy (analogous to heteronuclear NMR).
  • Assessment using simulated and experimental one- and two-color data.

Main Results:

  • The iterative algorithm successfully recovers relative phase relationships.
  • The technique provides access to multidimensional modalities beyond interferometric limitations.
  • Results compare favorably with solutions obtained through independent interferometry.

Conclusions:

  • The presented phase retrieval algorithm is effective and accurate for multidimensional optical spectroscopy.
  • Identified limitations and potential pitfalls require consideration for practical implementation.
  • Iterative phase retrieval algorithms hold potential for future advances in spectroscopy.