Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revisiting the Immunological Landscape of Locoregional Therapies for Gastrointestinal Cancers: A Shift Toward Interventional Immuno-Oncology.

Current oncology reports·2026
Same author

Correction: Bahloul et al. Investigating the Wound-Healing Potential of a Nanoemulsion-Gel Formulation of <i>Pituranthos tortuosus</i> Essential Oil. <i>Gels</i> 2024, <i>10</i>, 155.

Gels (Basel, Switzerland)·2026
Same author

Correction to "Three in One: <i>In Vitro</i> and <i>In Vivo</i> Evaluation of Anticancer Activity of a Theranostic Agent that Combines Magnetic Resonance Imaging, Optical Bioimaging, and Photodynamic Therapy Capabilities".

ACS applied bio materials·2026
Same author

The tRNA moieties of both aminoacyl-tRNA substrates of a cyclodipeptide synthase share a common binding site, as revealed by RNA microhelices mimicking tRNA acceptor arms.

Nucleic acids research·2026
Same author

First preclinical evaluation of a thermogel delivering mitomycin C: sustained local release with preserved surgical safety in a large animal model.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2026
Same author

Pre-clinical evaluation of mRNA-lipid nanoparticles' potency and toxicity: current practices and future directions.

In vitro models·2026
Same journal

Hijacking radiotherapy-induced chemokines with self-assembled molecular decoys for glioblastoma radio-immunotherapy.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same journal

A one-step surface engineering strategy based on PEG-EGCG to improve systemic delivery and survival of transplanted mesenchymal stromal cells.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same journal

Selective silencing of placental anti-angiogenic factors in invasive trophoblasts via membrane-fusion divalent siRNA delivery for preeclampsia therapy.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same journal

Unified inactivation-mineralization: An engineered bacterial platform for synergistic radio-immunotherapy.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same journal

Loss of internal structural order induced by freezing and Lyophilization correlates with reduced in vitro activity of mRNA lipid nanoparticles.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same journal

Muscle contribution in lipid nanoparticle mediated mRNA vaccine delivery and efficacy.

Journal of controlled release : official journal of the Controlled Release Society·2026
See all related articles

Related Experiment Video

Updated: Jun 5, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by &#960;-&#960; Stacking Interactions
10:53

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions

Published on: October 10, 2016

A comprehensive study in triblock copolymer membrane interaction.

Gaëlle Pembouong1, Nelly Morellet, T Kral

  • 1Laboratoire de Pharmacologie Chimique et Génétique et d'Imagerie, UMR 8151 CNRS, U 1022 INSERM, Faculté des Sciences Pharmaceutiques et Biologiques, 4, Avenue de l'Observatoire, 75270 Paris Cedex 06, France.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|January 19, 2011
PubMed
Summary
This summary is machine-generated.

Poloxamer copolymers do not interact with DNA, but their hydrophobic segments deeply interact with lipid membranes. This interaction destabilizes membranes, facilitating small molecule release and impacting gene transfer.

More Related Videos

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies
07:31

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

Published on: September 1, 2023

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Related Experiment Videos

Last Updated: Jun 5, 2026

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by &#960;-&#960; Stacking Interactions
10:53

Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions

Published on: October 10, 2016

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies
07:31

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

Published on: September 1, 2023

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Area of Science:

  • Biochemistry
  • Materials Science
  • Molecular Biology

Background:

  • Poloxamers, triblock copolymers, enhance gene transfer, suggesting polymer-DNA interaction.
  • Understanding poloxamer interaction with membranes is crucial due to their hydrophobic core.
  • The mechanism of poloxamer interaction (spanning vs. adsorption) with membranes remains unclear.

Purpose of the Study:

  • To investigate poloxamer (L64) interactions with DNA and lipid membranes.
  • To elucidate the role of poloxamer-membrane interactions in gene transfection.

Main Methods:

  • Time-correlated single-photon counting and fluorescence correlation spectroscopy.
  • (1)H NMR with doxyl probes and differential scanning calorimetry (DSC).
  • Molecular dynamics simulations.

Main Results:

  • Poloxamer L64 did not alter DNA diffusion or PicoGreen lifetime, indicating no direct DNA interaction.
  • Polypropylene glycol segments interacted deeply with lipid micelles and bilayers, influenced by cholesterol content.
  • Membrane destabilization and small molecule release were observed, correlating with molecular dynamics simulations.
  • Simulations showed poloxamer L64 hydrophobic core formed tight clusters, excluding membrane spanning.

Conclusions:

  • Poloxamers do not directly interact with DNA.
  • Poloxamer-membrane interactions, particularly with the hydrophobic core, are key to their function.
  • Membrane destabilization by poloxamers influences their efficacy in applications like gene transfer.