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Related Experiment Video

Updated: Jun 5, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

Published on: December 4, 2021

A microfabricated graphitic carbon column for high performance liquid chromatography.

D A Barrow¹, O K Castell, N Sykes

¹Cardiff School of Engineering, Cardiff University, 1-5 The Parade, Cardiff CF24 3AA, UK. barrow@cf.ac.uk

Journal of Chromatography. A

|January 19, 2011

Summary

Researchers developed a novel microfluidic graphitic carbon column with diamond-shaped micropillars for separations. This innovative chromatography device shows potential for advanced analytical applications, including subcritical water chromatography.

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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

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Published on: April 26, 2016

Area of Science:

Analytical Chemistry
Materials Science
Microfluidics

Background:

Traditional chromatography often relies on packed beds of porous materials, which can have limitations in terms of pressure and temperature stability.
Developing novel stationary phases and microfluidic formats is crucial for advancing separation science and analytical techniques.

Purpose of the Study:

To report the first development of a planar, microfluidic, graphitic carbon separations column.
To utilize graphitic micropillars of diamond cross-section as a monolithic chromatographic stationary phase.
To demonstrate the potential of this novel separations format for analytical applications.

Main Methods:

Fabrication of a planar, graphitic substrate with integrated fluidic architectures and a micropillared array using femtosecond laser ablation.
Integration of an on-chip sample injector and fluid-flow distribution systems to minimize band-broadening.
Direct interfacing of the microfluidic chip with an Electrospray Ionization (ESI) mass spectrometer for analyte detection.

Main Results:

Successful fabrication of a monolithic microfluidic graphitic carbon column with diamond-shaped micropillars.
Demonstration of differential analyte retention and separation on the micro-pillared graphitic column.
Detection of test-mixture analytes via direct coupling to mass spectrometry.

Conclusions:

The developed microfluidic graphitic carbon column represents a novel and promising separations format.
The monolithic design and graphitic micropillar stationary phase offer potential advantages in terms of temperature and pressure resilience.
This technology holds potential for applications in subcritical water chromatography and other advanced analytical separations.