Monosaccharide-NAIM derivatives for D-, L-configurational analysis

Area of Science:

• Analytical Chemistry
• Carbohydrate Chemistry

Background:

• Accurate analysis of monosaccharide composition and configuration is crucial in biochemistry and medicine.
• Existing methods for saccharide analysis can be time-consuming and complex.

Purpose of the Study:

• To develop a rapid and efficient capillary electrophoresis (CE) method for analyzing saccharide composition and configuration.
• To establish a derivatization strategy using 2,3-naphthalenediamine for monosaccharide analysis.

Main Methods:

• Derivatization of D-, L-enantiomeric monosaccharides with 2,3-naphthalenediamine to form aldo-NAIM derivatives.
• Capillary electrophoresis (CE) using borate buffer for simultaneous migration time determination.
• Enantioseparation using modified sulfated-α-cyclodextrin as a chiral selector.
• Diastereomeric pair formation for saccharide configuration analysis via reductive amination.

Main Results:

• A facile CE method for sugar composition analysis with quantitation levels of 10–500 ppm and LOD of 1 ppm.
• Successful enantioseparation of D, L aldo-NAIM pairs.
• Demonstrated applicability to various monosaccharides, including sialic acids.
• Aldo-NAIM derivatives enable rapid analysis with good linearity and short run times.

Conclusions:

• Aldo-NAIM derivatives are effective reagents for comprehensive saccharide analysis using CE.
• The developed CE method provides a sensitive and efficient approach for both composition and configuration determination.
• This methodology holds promise for applications in glycobiology and related fields.