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Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

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Related Experiment Video

Updated: Jun 5, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Epigenetics: Tools and Technologies.

William P Janzen¹, Tim J Wigle, Jian Jin

¹The Center for Integrative Chemical Biology and Drug Discovery, The University of North Carolina at Chapel Hill, Genetics Medicine Building, Campus Box 7363, Chapel Hill, NC 27599-7363, USA.

Drug Discovery Today. Technologies

|January 19, 2011

Summary

This summary is machine-generated.

Epigenetics involves heritable changes controlling genome access, enabling cell specialization. Understanding epigenetic

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Last Updated: Jun 5, 2026

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Published on: April 5, 2018

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Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

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Published on: December 10, 2014

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Epigenetics governs gene expression without altering DNA sequence.
Cellular identity arises from differential gene access, orchestrated by epigenetics.
Chromatin structure, regulated by histone modifications, dictates genome accessibility.

Purpose of the Study:

To elucidate the mechanisms of epigenetic regulation.
To highlight the role of histone modifications in cell differentiation.
To identify key players in the epigenetic machinery for therapeutic targeting.

Main Methods:

Focus on histone modifications and DNA methylation.
Analysis of chromatin structure and protein interactions.
Investigating the function of epigenetic enzymes (writers, readers, erasers).

Main Results:

Specific histone marks and DNA modifications define cell-specific gene expression.
Proteins recognizing these marks (readers) interpret the epigenetic information.
Enzymes (writers and erasers) dynamically regulate the epigenetic landscape.

Conclusions:

Epigenetic mechanisms are crucial for cell specialization and development.
The interplay of writers, readers, and erasers constitutes the 'histone code'.
Targeting epigenetic enzymes offers potential for treating human diseases.