Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe
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Published on: January 5, 2015
Carolina D Andrade1, Ciceron O Yanez, Maher A Qaddoura
1Department of Chemistry, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816, USA.
Abstract:
We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.
