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MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
Cardiomyopathy V: Interprofessional Care01:29

Cardiomyopathy V: Interprofessional Care

Managing cardiomyopathy involves addressing underlying or precipitating causes, treating heart failure with medications, and implementing dietary changes and a balanced exercise and rest regimen.Lifestyle ModificationsCardiomyopathy patients should adopt a low-sodium diet to reduce fluid retention and manage heart failure. A personalized exercise and rest plan helps maintain physical fitness without overstraining the heart. Avoiding alcohol and tobacco is essential to prevent further damage to...
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...

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Related Experiment Video

Updated: Jun 5, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

Cardioprotective microRNAs.

Maha Abdellatif1

  • 1Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA. abdellma@umdnj.edu

Pediatric Cardiology
|January 19, 2011
PubMed
Summary

Rapid changes in microRNA (miRNA) levels, like miR-21 and miR-199a-5p, are linked to cell signaling pathways such as AKT. This suggests posttranscriptional regulation fine-tunes gene expression during transient signaling events.

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Genetics

Background:

  • MicroRNAs (miRNAs) regulate diverse cellular functions, but their role in rapid cell signaling is not well understood.
  • The v-akt murine thymoma viral oncogene homolog 1 (AKT) pathway is known to influence cell processes.
  • Transient signaling events often require rapid cellular responses.

Purpose of the Study:

  • To investigate the role of microRNAs in immediate cell signaling.
  • To explore how signaling pathways like AKT rapidly modulate miRNA levels.
  • To understand the mechanisms behind rapid miRNA fluctuations.

Main Methods:

  • Observational study analyzing miRNA expression levels.
  • Investigating the relationship between AKT pathway activation/inhibition and specific miRNA abundance.

More Related Videos

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Related Experiment Videos

Last Updated: Jun 5, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

  • Examining posttranscriptional regulation of miRNA processing and stability.

    • Main Results:

    • Rapid changes in miR-21 and miR-199a-5p levels were observed within minutes of AKT pathway modulation.
    • AKT pathway activation led to increased miR-21 and decreased miR-199a-5p.
    • Evidence suggests posttranscriptional mechanisms are responsible for these rapid miRNA fluctuations.

    Conclusions:

    • Signaling pathways, such as AKT, can rapidly modulate miRNA levels through posttranscriptional regulation.
    • These rapid miRNA changes allow for prompt modulation of gene translation, bypassing transcriptional control.
    • This mechanism provides a way for cells to respond quickly to transient signaling events.