Related Experiment Video
Updated: Jun 5, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
Published on: April 29, 2022
Less label, more free: approaches in label-free quantitative mass spectrometry
Karlie A Neilson1, Naveid A Ali, Sridevi Muralidharan
1Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia.
Abstract:
In this review we examine techniques, software, and statistical analyses used in label-free quantitative proteomics studies for area under the curve and spectral counting approaches. Recent advances in the field are discussed in an order that reflects a logical workflow design. Examples of studies that follow this design are presented to highlight the requirement for statistical assessment and further experiments to validate results from label-free quantitation. Limitations of label-free approaches are considered, label-free approaches are compared with labelling techniques, and forward-looking applications for label-free quantitative data are presented. We conclude that label-free quantitative proteomics is a reliable, versatile, and cost-effective alternative to labelled quantitation.
Related Concept Videos
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry
Mass Spectrometry: Overview
Mass Analyzers: Overview
Mass Spectrometers
Mass Spectrum: Interpretation

