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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

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Related Experiment Video

Updated: Jun 5, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantitative proteomics for epigenetics.

H Christian Eberl¹, Matthias Mann, Michiel Vermeulen

¹Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.

Chembiochem : a European Journal of Chemical Biology

|January 19, 2011

Summary

This summary is machine-generated.

Mass spectrometry, a powerful tool in epigenetics research, now enables the comprehensive mapping and quantification of histone modifications and protein interactions. This advanced proteomics technology provides deep insights into gene regulation and cellular responses.

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Last Updated: Jun 5, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Published on: November 15, 2017

Quantitative Analysis of Chromatin Proteomes in Disease

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Published on: December 28, 2012

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Mass spectrometry (MS) is crucial for chromatin research, aiding in histone modification mapping and identifying gene regulation complexes.
Proteomics has advanced from single protein identification to quantifying thousands of proteins in complex biological samples.
Quantitative proteomics enables comparative and time-resolved analyses of post-translational modifications.

Purpose of the Study:

To review modern quantitative proteomic technologies.
To highlight the applications of these technologies in the field of epigenetics.
To discuss emerging areas like unbiased interaction analysis.

Main Methods:

Utilizing advanced mass spectrometry-based proteomics.
Employing quantitative approaches for protein and modification analysis.
Investigating protein-protein, protein-DNA, and protein-RNA interactions.

Main Results:

Identification and quantification of thousands of proteins in complex mixtures.
Comparative and time-resolved analysis of post-translational modifications.
Monitoring of whole proteome changes in response to gene expression perturbations.

Conclusions:

Quantitative proteomics offers powerful tools for epigenetic research.
The technology facilitates unbiased interaction analysis and comprehensive proteome monitoring.
Modern MS-based proteomics significantly advances our understanding of gene regulation.