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[Determination of GATA-4 in the testis of the mouse]
Jia-Zhou Yang1, Yu-Feng Zhao, Yan-Mei Wang
1Department of Human Histology and Embryology, Yan'an University School of Medicine, Yan'an, Shaanxi 716000, China. jiazhouy@yahoo.com.cn
Objective:
To explore the characteristics and distribution of GATA-4 in the testis of male mice.
Methods:
Paraffin sections were obtained from the testes of 24 male B6SJLF1/J mice, aged 0 day (n = 6), 2 weeks (n = 6), 4 weeks (n = 6) and 6 weeks (n = 6), and the expressions of GATA-4 in the testis were observed by the immunohistochemical ABC method and DAB visualization at different times.
Results:
Positive expressions of GATA4 were found in the Sertoli cells and Leydig cells of all the mice, but significantly higher in the 4- and 6-week-old than in the 0-day and 2-week-old groups (P < 0.01). And they were also observed in the germ cells of the 4- and 6-week-old mice, significantly higher in the latter than in the former (P < 0.01).
Conclusion:
GATA-4 exists in the testis of male mice, which has provided a morphological base for sex determination and differentiation and hormone regulation in the testis.
