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[Expression of SOCS3 gene and its effect on cell proliferation in human lung adenocarcinoma cell line A549]
1Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R.China.
Background:
To investigate the expression of the SOCS3 gene and its effect on proliferation of A549 cells.
Methods:
A549 cells were cotransfected with pEFSOCS3 and pSV2neo by liposome, then G418 was used to screen the positive cells. Expression of SOCS3 mRNA and protein was detected by RT-PCR and immunocytochemistry respectively before and after transfection. MTT assay was used to detect the cell growth. Flow cytometric DNA analysis was used to determine the cell cycle.
Results:
RT-PCR and immunocytochemistry showed that no expression of SOCS3 mRNA and protein was detected in A549 cells before transfection, but a stable expression of SOCS3 gene was observed after transfection with SOCS3 gene. Compared with control group, growth of A549 cells transfected with SOCS3 gene was significantly suppressed, with a suppressive rate of 41.07%. The cells at G₀/G₁ cell phases increased, and those at S and G₂/M phases decreased significantly after transfection.
Conclusions:
SOCS3 protein might inhibit the proliferation of A549 cells by negatively regulating cellular signal pathways.
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