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Related Concept Videos

Recombinant DNA01:09

Recombinant DNA

Overview
Recombinant DNA01:09

Recombinant DNA

Overview
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Jun 5, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Recombinant DNA and gene isolation.

J Robbins1

  • 1Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, OH 45267-0575, USA.

Trends in Cardiovascular Medicine
|January 20, 2011
PubMed
Summary
This summary is machine-generated.

Molecular genetics provides a powerful tool to study heart development and function by examining gene sequences and regulation. This approach complements traditional cardiovascular research, offering a genetic perspective on cardiac health and disease.

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Area of Science:

  • Cardiovascular Research
  • Molecular Genetics
  • Developmental Biology

Background:

  • Traditional cardiovascular research often employs systemic and integrative approaches.
  • Molecular genetics offers a reductionist perspective on gene sequences and transcripts.
  • Understanding organ development and function benefits from studying underlying genetic complements.

Purpose of the Study:

  • To highlight the complementary role of molecular genetics in cardiovascular research.
  • To define normal and abnormal heart functions in terms of genetic regulation.
  • To explore the application of molecular genetics to study heart development and function.

Main Methods:

  • Utilizing molecular genetics and recombinant DNA technologies.
  • Applying a reductionist approach to analyze gene sequences and transcripts.
  • Investigating genetic components involved in heart development and function.

Main Results:

  • Molecular genetics enables defining heart function based on genetic complement and regulation.
  • This approach allows for the study of genetic underpinnings of normal and abnormal cardiac processes.
  • The technology facilitates understanding gene sequences and transcripts related to organ development.

Conclusions:

  • Molecular genetics provides a complementary focus for cardiovascular research.
  • Defining heart function through genetic components is a key application.
  • Integrating genetic information with intact biological system function is a future challenge.