Recombinant DNA and gene isolation

Although a formidable array of approaches fall under the broad umbrella of molecular genetics, the basic technology is elegant and conceptually simple (Watson et al. 1983, Roberts et al. 1992). It has enabled a reductionist approach to be applied to the study of genes and their sequences, as well as the transcripts that underlie organ development and function. Cardiovascular research has, in the past, utilized the systemic and integrative approaches that are inherent in the study of an organ system. Molecular genetics and recombinant DNA offer the cardiologist a complementary focus, as both normal and abnormal heart functions can be defined in terms of the underlying genetic complement and its regulation. One can approach heart function in terms of defining the basic components that participate in the developmental and functional processes of the heart at different developmental times. A future challenge will be to integrate the information regarding these different components with the heart's function in an intact biological system.