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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 5, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

High-spatial-resolution sub-surface imaging using a laser-based acoustic microscopy technique.

Oluwaseyi Balogun, Garrett D Cole, Robert Huber

    IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
    |January 20, 2011
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a laser-based acoustic microscopy technique for high-resolution imaging of solid materials. This method overcomes limitations of traditional techniques by eliminating coupling media, enabling deeper interior imaging with micrometer precision.

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    Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography
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    Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography

    Published on: May 15, 2017

    Area of Science:

    • Materials Science
    • Non-destructive Testing
    • Acoustic Microscopy

    Background:

    • Scanning acoustic microscopy (SAM) offers micrometer-scale resolution for elastic characterization and interior imaging of solids.
    • High-frequency acoustic wave propagation is limited by energy losses in coupling media, restricting interrogation depth.
    • Traditional SAM requires coupling media, which attenuates ultrasound and limits penetration depth.

    Discussion:

    • A novel laser-based acoustic microscopy (LAM) technique is presented, utilizing pulsed laser generation of broadband acoustic waves and optical interferometric detection.
    • The system employs a 900-ps microchip pulsed laser, generating acoustic waves up to 1 GHz for micrometer-scale feature resolution.
    • Eliminating ultrasonic coupling media through optical generation and detection enhances penetration depth to several hundred micrometers.

    Key Insights:

    • Laser-based acoustic microscopy achieves micrometer-scale spatial resolution for elastic characterization and interior imaging.
    • The elimination of coupling media significantly improves penetration depth compared to conventional SAM.
    • The technique successfully imaged subsurface features in a silicon wafer, demonstrating its practical application.

    Outlook:

    • Further development of LAM could enable advanced non-destructive evaluation of microstructures.
    • This technique holds potential for applications in semiconductor inspection and materials science research.
    • Future work may focus on extending penetration depth and refining imaging capabilities for complex materials.