Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phospholipids of a bone matrix calcification nucleator.

J Ennever, J J Vogel, L J Riggan

    Journal of Dental Research
    |May 1, 1978
    PubMed
    Summary

    Researchers identified specific phospholipids, including phosphoinositides and phosphatidyl serine, within the bone matrix calcification nucleator. These findings advance our understanding of bone formation mechanisms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    'A time bomb ticking in my head': drawings of inner ears by patients with vestibular schwannoma.

    Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2011
    Same author

    Pathogenicity of the pulpless tooth.

    Washington University dental journal·2010
    Same author

    Redesigning nucleic acids.

    Pure and applied chemistry. Chimie pure et appliquee·2001
    Same author

    1,25 dihydroxyvitamin D(3) activates sphingomyelin turnover in ROS17/2.8 osteosarcoma cells without sphingolipid-induced changes in cytosolic Ca(2+).

    Biochemical and biophysical research communications·2000
    Same author

    Fingertip necrosis during chemotherapy with bleomycin, vincristine and methotrexate for HIV-related Kaposi's sarcoma.

    The British journal of dermatology·1996
    Same author

    Mineralization of bone-like extracellular matrix in the absence of functional osteoblasts.

    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·1995

    Area of Science:

    • Biochemistry
    • Bone Biology
    • Cellular Biophysics

    Background:

    • Bone matrix calcification is a critical process for skeletal integrity.
    • The precise molecular components initiating calcification remain incompletely understood.
    • Protein-lipid complexes are implicated as potential nucleation sites.

    Purpose of the Study:

    • To identify the specific phospholipid components of the bone matrix calcification nucleator.
    • To characterize the composition of the protein-phospholipid complex involved in bone formation.

    Main Methods:

    • Dissociation of the protein-phospholipid complex using acidified-solvent porous-glass column chromatography.
    • Identification and analysis of phospholipids via gas-liquid chromatography.

    Main Results:

    • Mono- and diphosphoinositides were identified as key phospholipid components.
    • Phosphatidyl serine was also identified as a significant phospholipid in the nucleator.
    • The study successfully dissociated the protein-phospholipid complex for analysis.

    Conclusions:

    • Mono- and diphosphoinositides and phosphatidyl serine are integral phospholipids of the bone matrix calcification nucleator.
    • These phospholipids likely play a crucial role in initiating hydroxyapatite formation.
    • The findings provide molecular insights into the early stages of biomineralization.

    Related Experiment Videos