Related Experiment Video
Updated: Jun 5, 2026
HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Simultaneous determination of rutin, isoquercitrin and chlorogenic acid in Farfarae Flos by HPLC]
Di Wu1, Zhengtao Wang, Mian Zhang
1Department of Pharmacognosy, China Pharmaceutical University, Nanjing 211198, China. michael2141@hotmail.com
Objective:
To develop an HPLC method for simultaneous determination of rutin, isoquercitrin and chlorogenic acid in Farfarae Flos.
Method:
The analysis was carried out on a Phenomenex Synergi POLAR-RP 80A column (4.6 mm x 250 mm, 4 microm) with gradient elution using methanol-acetonitrile-water (adjusted to pH 2.5 with formic acid) as mobile phase. The flow rate was 1.2 mL x min(-1) and the detection wavelength was at 255 nm.
Result:
The calibration curves were linear over the range of 0.2-2 000 microg x L(-1) for rutin and isoquercitrin, 10-2 000 microg x L(-1) for chlorogenic acid, respectively. The average recoveries were 99.5% for rutin, 100.1% for isoquercitrin and 99.4% for chlorogenic acid, respectively, with RSD not more than 3.0%.
Conclusion:
The described method is reliable and could be used for the quality control of Farfarae Flos.
