Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanoparticle delivery systems for HIV pre-exposure prophylaxis (PrEP): advances and challenges.

Beilstein journal of nanotechnology·2026
Same author

Ultrafast-light driven plasmonic inactivation of <i>S. epidermidis</i>: mechanistic insights.

Journal of materials chemistry. B·2026
Same author

Assembly and Stability of Lipid-Coated Silver Nanoparticles: A Combined UV-VIS / Multispectral iSCAT Study.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

Detecting conformational changes in switchable heterodimer plasmon rulers through iSCAT fluctuation microscopy.

Nanoscale horizons·2026
Same author

Plasmon-enhanced photocatalytic nanoreactors for selective inactivation of murine leukemia virus (MLV).

Nanoscale advances·2025
Same author

Enhancing antiretroviral delivery to secondary lymph nodes by targeting CD169 + macrophages with HIV-mimicking nanoparticles.

Scientific reports·2025

Related Experiment Video

Updated: Jun 5, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Optical sizing of immunolabel clusters through multispectral plasmon coupling microscopy.

Hongyun Wang1, Guoxin Rong, Bo Yan

  • 1Department of Chemistry, Boston University, Boston, Massachusetts 02215, USA.

Nano Letters
|January 21, 2011
PubMed
Summary

Researchers developed a method to measure silver nanoparticle cluster size using light scattering. This technique can map nanoparticle association levels on cellular surfaces for biological studies.

More Related Videos

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jun 5, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

  • Nanotechnology
  • Optical Physics
  • Biophysics

Background:

  • Silver nanoparticles exhibit unique optical properties dependent on their size and aggregation state.
  • Accurate characterization of nanoparticle clusters is crucial for their application in sensing and imaging.

Purpose of the Study:

  • To develop and validate an optical method for determining the size and association levels of silver nanoparticle clusters.
  • To demonstrate the feasibility of using this method for mapping immunolabel distribution on cell surfaces.

Main Methods:

  • Measurement of wavelength-dependent scattering cross sections of silver nanoparticle clusters using multispectral imaging.
  • Correlation of spectral response with cluster size via multivariate statistical analysis.
  • Application of the developed method to silver immunolabels targeting epidermal growth factor receptor on A431 cells.

Main Results:

  • A robust correlation was established between the spectral response and silver nanoparticle cluster size.
  • A classification system (I1-I4) was developed to categorize clusters based on association levels (monomers to larger aggregates).
  • The optical sizing approach was successfully demonstrated on living cells, mapping immunolabel association levels.

Conclusions:

  • Optical sizing of silver nanoparticle clusters is feasible and provides a non-invasive method for assessing size and association.
  • This technique enables the measurement of immunolabel association levels on subcellular scales.
  • The approach offers new possibilities for studying receptor density distributions on living cells.