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Related Experiment Video

Updated: Jun 5, 2026

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Lipolytic function of adipocyte/endothelial cocultures.

Jennifer H Choi1, Evangelia Bellas, Jeffrey M Gimble

  • 1Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA.

Tissue Engineering. Part A
|January 21, 2011
PubMed
Summary

This study developed a novel in vitro adipose tissue model using silk fibroin scaffolds. The engineered tissue successfully mimics in vivo metabolic functions, offering a new tool for studying obesity and related disorders.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Metabolic Research

Background:

  • Rising incidence of adipose-related disorders like obesity necessitates advanced in vitro models.
  • Functional human soft tissues in vitro are crucial for disease research and treatment development.
  • In vitro tissues mimicking in vivo structure and metabolic function offer insights into adipose tissue development.

Purpose of the Study:

  • To investigate the metabolic potential of adipose/endothelial cocultures on 3D silk fibroin scaffolds.
  • To evaluate endothelial contributions to adipose lipogenesis and lipolysis in a coculture system.
  • To assess the viability of developing a tissue-engineered adipose tissue with both structural and metabolic functions.

Main Methods:

  • Utilized three-dimensional silk fibroin scaffolds for adipose/endothelial cocultures.

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  • Measured triglyceride accumulation and adipogenic gene transcript expression.
  • Assessed basal lipolysis in both monoculture and coculture systems.

    • Main Results:

    • Coculture system demonstrated retention of metabolic levels comparable to adipocyte monocultures.
    • Triglyceride accumulation and adipogenic gene expression were maintained.
    • Basal lipolysis was significantly stimulated in both mono- and cocultures, with cocultures showing a 1.9-fold increase.

    Conclusions:

    • The developed coculture system successfully retains key adipose functions.
    • This represents a significant advancement in creating tissue-engineered adipose tissue with endothelial lumens and metabolic capabilities.
    • The model provides a valuable platform for studying adipose tissue development and disease mechanisms.