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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

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Related Experiment Video

Updated: Jun 5, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Large-scale prediction of long non-coding RNA functions in a coding-non-coding gene co-expression network.

Qi Liao1, Changning Liu, Xiongying Yuan

  • 1Bioinformatics Research Group, Key Laboratory of Intelligent Information Processing, Advanced Computing Research Laboratory, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, PR China.

Nucleic Acids Research
|January 21, 2011
PubMed
Summary

This study introduces the first computational method to predict long-non-coding RNA (lncRNA) functions using gene expression data. It identified probable functions for 340 lncRNAs involved in development, transport, and metabolism.

Related Experiment Videos

Last Updated: Jun 5, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Area of Science:

  • Computational Biology
  • Genomics
  • Bioinformatics

Background:

  • Long-non-coding RNAs (lncRNAs) play crucial roles in biological processes, but their functions remain largely uncharacterized.
  • Existing research has illuminated some lncRNA functions, yet a significant portion of these transcripts lack defined roles.

Purpose of the Study:

  • To develop and apply a computational approach for annotating the functions of uncharacterized lncRNAs.
  • To predict probable functions for a large set of lncRNAs using public microarray expression data.

Main Methods:

  • Construction of a coding-non-coding gene co-expression (CNC) network using re-annotated Affymetrix Mouse Genome Array data.
  • Prediction of lncRNA functions based on network characteristics, including module sharing, association with network hubs, and co-expression with genomic adjacency.

Main Results:

  • Successfully annotated probable functions for 340 lncRNAs.
  • Identified key functional categories including organ/tissue development (e.g., neuronal, ocular, muscular), cellular transport (e.g., neuronal, ion, lipid), and metabolic processes (e.g., macromolecule, phosphocreatine, tyrosine).

Conclusions:

  • This study presents the first computational framework for lncRNA function annotation.
  • The findings provide valuable insights into the roles of numerous lncRNAs in fundamental biological processes, paving the way for future experimental validation.