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Protective effect of Cassia fistula fruit extract on bromobenzene-induced nephrotoxicity in mice
Heibatullah Kalantari1, Mohammadtaha Jalali, Amir Jalali
1School of Pharmacy, Toxicology Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran.
Abstract:
The efficacy of a crude hydro-alcoholic extract of Cassia fistula (golden shower tree) fruit to protect the kidney against bromobenzene-induced toxicity was studied. Negative control mice received normal saline; positive control mice were given 460 mg/kg of bromobenzene; Cassia fistula treated mice received 200, 400, 600 and 800 mg/kg of Cassia fistula fruit extract followed by 460 mg/kg bromobenzene (daily by oral gavage for 10 days). On the 11th day, the mice were sacrificed, blood samples were obtained to assess blood urea nitrogen (BUN) and creatinine levels, and kidneys were removed for histological examination. We found that bromobenzene induced significant nephrotoxicity reflected by an increase in levels of BUN and creatinine that was dose dependently prevented by the Cassia fistula fruit extract. The nephroprotective effect of the Cassia fistula fruit extract was confirmed by the histological examination of the kidneys. To the best of our knowledge, this is the first study to demonstrate the protective effect of Cassia fistula in nephrotoxicity.
Insights
Cassia fistula fruit extract protected kidneys from bromobenzene toxicity. This study shows the golden shower tree
Area of Science:
- Pharmacology
- Toxicology
- Natural Products Chemistry
Background:
- Kidney damage from toxins like bromobenzene is a significant health concern.
- Natural products are increasingly investigated for therapeutic potential.
Purpose of the Study:
- To evaluate the nephroprotective effects of Cassia fistula fruit extract against bromobenzene-induced kidney toxicity.
- To determine the dose-dependent efficacy of the extract.
Main Methods:
- A crude hydro-alcoholic extract of Cassia fistula fruit was prepared.
- Mice were treated with varying doses of the extract followed by bromobenzene administration.
- Kidney function was assessed by measuring blood urea nitrogen (BUN) and creatinine levels.
- Kidney tissues were examined histologically.
Main Results:
- Bromobenzene significantly increased BUN and creatinine levels, indicating nephrotoxicity.
- Cassia fistula extract dose-dependently reduced these elevated levels.
- Histological analysis confirmed the protective effect of the extract on kidney tissue.
Conclusions:
- Cassia fistula fruit extract exhibits significant nephroprotective properties against bromobenzene toxicity.
- This study provides the first evidence of Cassia fistula's protective role in nephrotoxicity.
