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Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
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Published on: March 17, 2016

Estrogen receptor-β signaling modulates epithelial barrier function.

Mirjam Looijer-van Langen1, Naomi Hotte, Levinus A Dieleman

  • 1Division of Gastroenterology, Department of Medicine, University of Alberta, Edmonton, Canada.

American Journal of Physiology. Gastrointestinal and Liver Physiology
|January 22, 2011
PubMed
Summary

Reduced estrogen receptor-beta (ERβ) expression is linked to increased intestinal permeability in inflammatory bowel disease (IBD). This study highlights ERβ signaling

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Area of Science:

  • Gastroenterology
  • Endocrinology
  • Molecular Biology

Background:

  • Estrogen receptor-beta (ERβ) is crucial for colon health, with its absence linked to disrupted epithelial barriers.
  • Impaired epithelial barrier function and estrogen signaling are implicated in inflammatory bowel disease (IBD) pathogenesis.

Purpose of the Study:

  • To investigate the role of ERβ signaling in maintaining colonic epithelial permeability.
  • To assess ERβ expression and its correlation with barrier function in IBD models and patients.

Main Methods:

  • Assessed ERβ mRNA and colonic permeability in IL-10-deficient mice and HLA-B27 rats using RT-PCR and Ussing chambers.
  • Measured ERβ expression and monolayer resistance in HT-29 and T84 colonic cell lines via RT-PCR and electric cell-substrate impedance sensing.
  • Examined the effects of estradiol, DPN, and ICI 182780 on T84 cell resistance and analyzed ERβ expression in IBD patient biopsies.

Main Results:

  • IL-10-deficient mice and HLA-B27 rats showed decreased ERβ mRNA and increased colonic permeability before colitis onset.
  • T84 cells exhibited higher ERβ mRNA levels and greater resistance compared to HT-29 cells.
  • 17β-estradiol and DPN enhanced T84 cell resistance, an effect blocked by an ERβ antagonist; IBD patients displayed reduced ERβ mRNA in colonic tissue.

Conclusions:

  • ERβ signaling plays a significant role in modulating colonic epithelial permeability.
  • Reduced ERβ expression is observed in animal models of colitis and in the colons of IBD patients, suggesting its involvement in IBD.
  • Targeting ERβ signaling may offer a therapeutic strategy for IBD by improving intestinal barrier function.