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Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression

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RNA interference in mammalian cell systems.

Didier Lochmatter¹, Primus-E Mullis

¹Paediatric Endocrinology/Diabetology and Metabolism, University Children's Hospital, Inselspital, Bern, Switzerland.

Hormone Research in Paediatrics

|January 22, 2011

Summary

Small noncoding RNAs, including short interfering RNAs (siRNAs) and microRNAs (miRNAs), regulate gene expression and defend genomes. Recent discoveries reveal diverse biogenesis and regulatory mechanisms impacting fundamental biology and disease treatment.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

RNA molecular biology has undergone significant transformation in the past decade.
Small noncoding RNAs (20-30 nucleotides) are key regulators of genes and genomes.
RNA silencing and RNA interference encompass inhibitory mechanisms of small RNA action.

Purpose of the Study:

To review new techniques in molecular biology focusing on small RNAs.
To discuss the discovery and significance of short interfering RNAs (siRNAs) and microRNAs (miRNAs).
To explore the implications of siRNA and miRNA regulation for fundamental biology and disease.

Main Methods:

Review of recent advances in small RNA research.
Analysis of biogenesis pathways for siRNAs and miRNAs.
Examination of regulatory mechanisms employed by small RNAs.

Main Results:

Discovery of small noncoding RNAs (siRNAs and miRNAs) as crucial gene regulators.
Identification of diverse biogenesis pathways for siRNAs and miRNAs.
Understanding of inhibitory effects of small RNAs on gene expression.

Conclusions:

Small RNAs play vital roles in regulating endogenous genes and defending eukaryotic genomes.
Advances in understanding siRNA and miRNA mechanisms have broad implications for biology and medicine.
New techniques are enhancing our knowledge of RNA silencing and interference.