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GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
Gas chromatographic method for residue analysis of metsulfuron methyl from soil
Shashi Bala Singh1, Rupak Paul
1Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110012, India. sbs_agch@yahoo.com
Abstract:
Gas chromatographic method has been developed for the determination of metsulfuron methyl residues from soil. Due to the thermal unstability of this herbicide and its mono methyl derivative, it was derivatized to a dimethyl derivative using diazomethane. Structure of the derivatized product was confirmed by GC-MS. Time for methylation reaction was standardized and 24 h was found to be the optimum time in ethylacetate solvent. Using this method, recovery of metsulfuron methyl from soil was above 70%. Limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.1 μg mL⁻¹ and 0.2 μg g⁻¹ respectively.
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