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Updated: Jun 5, 2026
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
Published on: June 7, 2014
A silanized mica substrate suitable for high-resolution fiber FISH analysis by scanning near-field optical/atomic
Shigeru Sugiyama1, Megumi Fukuta, Tamaki Hirose
1Nanobiotechnology Laboratory, National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. ssugi@affrc.go.jp
Abstract:
We applied a novel silanized mica substrate with an extremely flat surface constructed according to Sasou et al. (Langmuir 19, 9845-9849 (2003)) to high-resolution detection of a specific gene on a DNA fiber by scanning near-field optical/atomic force microscopy (SNOM/AFM). The interaction between the substrate and fluorescence-dye conjugated peptide nucleic acid (PNA) probes, which causes fluorescence noise signal, was minimal. By using the substrate, we successfully obtained a fluorescence in situ hybridization signal from the ea47 gene on a λphage DNA labeled with an Alexa 532-conjugated 15-base PNA probe. As the results, no fluorescence noises were observed, indicating that the surface adsorbed almost none of the PNA probe. The combination of the substrate and SNOM/AFM is an effective tool for visualizing DNA sequences at nanometer-scale resolution.
