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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...

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Updated: Jun 5, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Bundled capillary electrophoresis using microstructured fibres.

Benjamin Rogers1, Graham T T Gibson, Richard D Oleschuk

  • 1Department of Chemistry, Queen's University, Kingston, ON, Canada.

Electrophoresis
|January 22, 2011
PubMed
Summary
This summary is machine-generated.

Microstructured fibers offer efficient capillary electrophoresis separations by mitigating Joule heating. This approach overcomes limitations of narrow capillaries, particularly for dye-labeled peptides.

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Last Updated: Jun 5, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Joule heating in capillary electrophoresis (CE) causes band broadening, limiting separation efficiency.
  • Narrow-bore capillaries reduce Joule heating but present challenges like low sensitivity and high backpressure.

Purpose of the Study:

  • To investigate the use of microstructured fibers as an alternative to conventional CE capillaries.
  • To leverage the high surface-to-volume ratio and small channel dimensions of microstructured fibers for improved separations.

Main Methods:

  • Utilized microstructured fibers, containing parallel microchannels, for capillary zone electrophoresis (CZE).
  • Compared performance against traditional CE techniques, focusing on separation efficiency for dye-labeled peptides.

Main Results:

  • Microstructured fibers effectively reduced negative impacts of Joule heating.
  • Achieved highly efficient separations, particularly for challenging samples like dye-labeled peptides.
  • Overcame limitations associated with single narrow-bore capillaries.

Conclusions:

  • Microstructured fibers are a promising alternative for CE, offering enhanced separation efficiency.
  • This technology addresses key challenges in CE, enabling better analysis of small peptides.