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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Jun 5, 2026

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
05:36

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery

Published on: October 4, 2024

An efficient binary system for gene expression in the silkworm, Bombyx mori, using GAL4 variants.

Isao Kobayashi1, Katsura Kojima, Keiro Uchino

  • 1Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

Archives of Insect Biochemistry and Physiology
|January 22, 2011
PubMed
Summary

Researchers improved the GAL4/UAS binary gene expression system in silkworms by testing new GAL4 variants. GAL4VP16 and GAL4NFκB showed high activity and toxicity, leading to a more efficient system for Bombyx mori research.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • The GAL4/UAS system is crucial for gene function analysis.
  • In Bombyx mori, GAL4/UAS utility is limited by low activity and toxicity.

Purpose of the Study:

  • To enhance the GAL4/UAS binary gene expression system in Bombyx mori.
  • To evaluate novel GAL4 variants for improved transcriptional activation and reduced toxicity.

Main Methods:

  • Constructed and tested various GAL4 variants (GAL4Δ, GAL4VP16, GAL4VPmad2, GAL4VPmad3, GAL4NFκB, GAL4Rel, GAL4Relish).
  • Assessed transactivation activity and toxicity in Bombyx mori cells, embryos, and transgenic silkworms.
  • Utilized constitutive and inducible promoters for GAL4 variant expression.

Main Results:

  • GAL4VP16 and GAL4NFκB demonstrated high transactivation but also toxicity.
  • GAL4VPmad2 and GAL4VPmad3 showed increased activity and toxicity compared to GAL4.
  • GAL4Δ exhibited doubled activity over GAL4, while GAL4Rel and GAL4Relish were less active.
  • GAL4VP16 and GAL4NFκB enabled a highly efficient binary gene expression system.

Conclusions:

  • Optimized GAL4 variants, particularly GAL4VP16 and GAL4NFκB, significantly improve the GAL4/UAS system in Bombyx mori.
  • The developed system is effective for gene expression studies in silkworm cells, embryos, and transgenic models.
  • This advancement facilitates deeper understanding of gene function in Bombyx mori.