Enzymatic properties of immobilized catalase on protein coated supports

Sephadex-100, DEAE-Sephadex and polyvinyl alcohol supports used for immobilization of catalase were prepared by modifications initially with 2-amino-4,6-dichloro-s-triazine and then with diaminohexane and glutaraldehyde prior to coating with gelatine, whereas the Fe2O3 support was coated directly. Optimum support/enzyme ratios were determined for these protein coated supports in a series of immobilization reactions with varying amounts of catalase in the presence of the crosslinking agent glutaraldehyde. Activity variations with respect to pH, temperature, stability behaviour and the inhibition effect of hydroxylamine were investigated for the immobilized catalase preparations. The time-dependent decomposition rate of H2O2 and the deactivation rate constants for catalase were investigated in a discontinuous batch-type reactor system.