Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 5, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
05:18

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

PAS/poly-HAMP signalling in Aer-2, a soluble haem-based sensor.

Kylie J Watts1, Barry L Taylor, Mark S Johnson

  • 1Division of Microbiology and Molecular Genetics, Loma Linda University, Loma Linda, CA 92350, USA. kwatts@llu.edu

Molecular Microbiology
|January 25, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uncovering Gaps in Obesity Medicine Competencies: Insights from Ten U.S. Medical Schools.

Medical science educator·2026
Same author

Spirituality as a Component of Student Resilience and Well-Being in Standards 2025.

American journal of pharmaceutical education·2026
Same author

Metabolism matters: Interspecies variability and ecological traits of biotransformation kinetics.

Ecotoxicology and environmental safety·2026
Same author

The Role of the Omega Subzone in Determining the Membership of a Protein in One of the Two Families of the LexA/Signal Peptidase-like Superfamily.

International journal of molecular sciences·2026
Same author

Novel, Stable, and Well-Secreted Single-Chain Fc Forms of Programmed Death-1 Immune Checkpoint Inhibitor Efficiently Block PD-1/PD-L1 Interaction.

Human gene therapy·2026
Same author

Recurrent cancer-associated ERBB4 mutations are transforming and confer resistance to targeted therapies.

Molecular oncology·2025
Same journal

Riboflavin Salvage Supports Glycolysis in Borrelia burgdorferi Through Flavin-Dependent NAD<sup>+</sup> Regeneration.

Molecular microbiology·2026
Same journal

Distinct Spatial Organisation of Rho and RNA Polymerase in Salmonella Cells.

Molecular microbiology·2026
Same journal

A Single-Nucleotide Substitution Generates a de Novo Promoter That Activates a Latent Metabolic Bypass in Escherichia coli.

Molecular microbiology·2026
Same journal

A Phosphorylation-Dependent Partner-Switching-Like Module Regulates a Glycosyltransferase Required for Heterocyst Polysaccharide Layer Formation in Anabaena sp. Strain PCC 7120.

Molecular microbiology·2026
Same journal

Chain-Length Regulation by WzzE Is Necessary for, but Genetically Separable From, Cyclic Enterobacterial Common Antigen Synthesis.

Molecular microbiology·2026
Same journal

To Move or Not to Move: When and How Bacteria Suppress Flagellar Motility.

Molecular microbiology·2026
See all related articles

Pseudomonas aeruginosa Aer-2, a chemoreceptor with multiple HAMP domains, mediates repellent responses. Ligand binding to the PAS domain and specific HAMP domains regulate its kinase activity.

Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • Poly-histidine-affinity-tag (HAMP) domains are common in bacterial chemoreceptors, but their function in multi-domain proteins is understudied.
  • The Pseudomonas aeruginosa chemoreceptor Aer-2 possesses a unique structure with five HAMP domains and a PAS sensing domain.

Purpose of the Study:

  • To investigate the signaling mechanism and poly-HAMP domain function of the Aer-2 chemoreceptor.
  • To elucidate the role of Aer-2 in Pseudomonas aeruginosa chemotaxis.

Main Methods:

  • Utilized a heterologous system by expressing Aer-2 in Escherichia coli to study its function.
  • Determined ligand binding properties of the Aer-2 PAS domain using spectroscopic methods.
  • Investigated the role of individual HAMP domains through deletion mutagenesis.

More Related Videos

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Related Experiment Videos

Last Updated: Jun 5, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
05:18

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Main Results:

  • Aer-2 mediates repellent responses to oxygen, carbon monoxide, and nitric oxide when reconstituted in E. coli.
  • The PAS domain of Aer-2 binds penta-coordinated b-type heme.
  • Reversible signaling necessitates four of the five HAMP domains, with specific N-terminal and C-terminal HAMP domains exhibiting opposing effects on kinase activity.

Conclusions:

  • A model is proposed where ligand-bound Aer-2 PAS and N-terminal HAMP domains (HAMP 2 and 3) relieve inhibition mediated by C-terminal HAMP domains (HAMP 4 and 5), activating the kinase.
  • This study provides novel insights into the structure-function relationship of poly-HAMP domains in bacterial chemoreception.