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(1→3)-β-D-Glucan inhibits a dual mechanism of peroxynitrite stroke
Joanna Saluk-Juszczak1, Karolina Krolewska, Barbara Wachowicz
1Department of General Biochemistry, University of Lodz, 90-237 Lodz, Banacha 12/16, Poland. juszczak@biol.uni.lodz.pl
Aim:
The antioxidative and antinitrative activities of (1→3)-β-D-glucan (1-4μg/ml) from the yeast cell walls of Saccharomyces cerevisiae in human plasma treated with strong oxidants - peroxynitrite (ONOO(-)) (0.1mM) and hydrogen peroxide (H(2)O(2)) (2mM) were studied in vitro. The main purpose of this study was to assess if (1→3)-β-D-glucan, a well known strong immunostimulatory agent, possesses a protective function against dual mechanism of ONOO(-) stroke associated with nitrative and oxidative damages to human plasma biomolecules.
Scope:
The protein changes were determined in vitro by estimating the level of oxidative stress markers - carbonyl groups, and nitrative products - 3-nitrotyrosine residues. The plasma lipid peroxidation was also investigated. The obtained results show that (1→3)-β-D-glucan inhibits in vitro ONOO(-)-induced oxidation and nitration of plasma proteins, even by 50% and 30%, respectively. The antioxidative activity of (1→3)-β-D-glucan was confirmed by its inhibitory effect on plasma lipids peroxidation induced by ONOO(-) or by H(2)O(2).
Conclusions:
The obtained results demonstrate that (1→3)-β-D-glucan from S. cerevisiae protects plasma components against toxic effects of ONOO(-) and H(2)O(2) due to its antioxidative and antinitrative activities. Therefore (1→3)-β-D-glucan supplementation during inflammatory may be beneficial not only regard for its ability to stimulate the immune system but also by antioxidative properties.
Insights
Saccharomyces cerevisiae (1→3)-β-D-glucan exhibits significant antioxidative and antinitrative effects. This yeast-derived compound protects human plasma from oxidative and nitrative damage caused by peroxynitrite and hydrogen peroxide.
Area of Science:
- Biochemistry
- Immunology
- Food Science
Background:
- Yeast (1→3)-β-D-glucan is a known immunostimulant.
- Oxidative and nitrative stress contribute to plasma biomolecule damage.
- Peroxynitrite (ONOO⁻) and hydrogen peroxide (H₂O₂) are potent oxidants.
Purpose of the Study:
- To investigate the antioxidative and antinitrative activities of (1→3)-β-D-glucan from Saccharomyces cerevisiae in human plasma.
- To determine if this glucan protects plasma components against peroxynitrite and hydrogen peroxide induced damage.
Main Methods:
- In vitro study using human plasma treated with peroxynitrite (ONOO⁻) and hydrogen peroxide (H₂O₂).
- Quantification of oxidative stress markers (carbonyl groups) and nitrative products (3-nitrotyrosine residues).
- Assessment of plasma lipid peroxidation levels.
Main Results:
- Saccharomyces cerevisiae (1→3)-β-D-glucan demonstrated significant inhibition of ONOO⁻-induced protein oxidation (up to 50%) and nitration (up to 30%).
- The glucan effectively inhibited plasma lipid peroxidation induced by both ONOO⁻ and H₂O₂.
- (1→3)-β-D-glucan showed protective effects against the toxic impacts of ONOO⁻ and H₂O₂ on plasma.
Conclusions:
- Saccharomyces cerevisiae (1→3)-β-D-glucan possesses notable antioxidative and antinitrative properties.
- It effectively protects human plasma components from oxidative and nitrative damage.
- Supplementation with this glucan may offer benefits during inflammation due to its immune-stimulating and antioxidative capacities.
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