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Human MxA protein inhibits the replication of classical swine fever virus

Yicheng Zhao1, Daxin Pang, Tiedong Wang

  • 1Department of Animal Biotechnology, Jilin Provincial Key Laboratory of Animal Embryo Engineering, The Center for Animal Embryo Engineering of Jilin Province, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, Jilin, PR China. zhao008@hotmail.com

Virus Research
|January 25, 2011
PubMed

Insights

Human MxA protein significantly inhibits classical swine fever virus (CSFV) replication. This finding reveals a potential antiviral strategy against CSFV, the cause of classical swine fever (CSF).

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Classical swine fever virus (CSFV) causes a severe contagious disease in pigs.
  • Interferon-induced MxA protein is known to inhibit certain RNA viruses.
  • CSFV reportedly blocks MxA expression, leaving its effect on CSFV unknown.

Purpose of the Study:

  • To investigate the inhibitory effect of human MxA protein on CSFV replication.
  • To determine if MxA can be a potential antiviral target against CSFV.

Main Methods:

  • Stable transfection of PK-15 cell lines and swine fetal fibroblasts (PEF) with human MxA.
  • Infection of MxA-expressing cells with CSFV.
  • Quantification of viral replication using virus titration, indirect immune fluorescence assay, and real-time PCR.

Main Results:

  • CSFV replicated poorly in cells stably expressing human MxA.
  • Proliferation of progeny CSFV was significantly inhibited in MxA-expressing PK-15 and PEF cells.
  • Virus titration, immune fluorescence, and real-time PCR confirmed the inhibitory effect.

Conclusions:

  • Human MxA protein possesses significant antiviral activity against CSFV.
  • MxA effectively inhibits CSFV replication in swine cells.
  • MxA represents a potential therapeutic target for controlling classical swine fever.

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