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Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Collection mode surface plasmon fibre sensors: a new biosensing platform.

A François1, J Boehm, S Y Oh

  • 1Institute for Photonics & Advanced Sensing, School of Chemistry & Physics, University of Adelaide, Adelaide, SA, Australia. alexandre.francois@adelaide.edu.au

Biosensors & Bioelectronics
|January 25, 2011
PubMed
Summary

This study introduces a novel surface plasmon resonance (SPR) biosensing method using light emission from silver-coated optical fibers. This technique offers improved sensitivity and multiplexing capabilities for detecting viruses like influenza A.

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A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
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A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
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A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

Published on: January 7, 2019

Area of Science:

  • Optoelectronics
  • Biosensing
  • Nanotechnology

Background:

  • Surface plasmon resonance (SPR) is a label-free optical biosensing technology.
  • Current SPR sensors infer plasmon existence indirectly through absorption.
  • Surface roughness can induce radiative SPR, emitting light.

Purpose of the Study:

  • To demonstrate a novel SPR transducing mechanism using light emission.
  • To explore advantages over traditional reflectance-based SPR measurements.
  • To enable multiplexed detection and combined sensing modalities.

Main Methods:

  • Utilizing silver-coated optical fibers for SPR.
  • Investigating light emission from surface roughness-induced plasmons.
  • Developing complex sensor architectures for multiplexing and self-referencing.
  • Integrating SPR with fluorescence sensing.

Main Results:

  • The novel SPR emission mechanism shows lower dependency on metallic thickness.
  • Higher signal-to-noise ratio compared to traditional reflectance methods.
  • Demonstrated multiplexed detection and dynamic self-referencing.
  • Successfully combined SPR and fluorescence sensing in a single device.
  • Preliminary detection of seasonal influenza A virus achieved.

Conclusions:

  • Radiative SPR from rough silver surfaces offers significant advantages for biosensing.
  • The developed fiber-optic platform enables advanced multiplexed and hybrid sensing.
  • This approach holds promise for sensitive and versatile pathogen detection, including influenza A.