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Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window
Published on: May 6, 2021
A novel in vitro pancreatic carcinogenesis model
Hyo Jin Kang1, Young Bin Hong, Hee Jeong Kim
1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Road, NW, Washington, DC 20057-1469, USA.
Toxicology Letters
|January 25, 2011
Summary
This study developed an in vitro model for pancreatic cancer research. BRCA1 knockdown cells showed increased benzo[a]pyrene (BaP)-DNA adducts, suggesting BRCA1
Area of Science:
- Biochemistry
- Molecular Biology
- Cancer Research
Background:
- Environmental factors like benzo[a]pyrene (BaP) are implicated in pancreatic cancer etiology.
- Limited experimental models exist to study pancreatic-specific environmental mutagens and susceptibility genes.
Purpose of the Study:
- To develop an in vitro cell culture model for studying pancreatic ductal cell carcinogenesis.
- To investigate the role of AhR and BRCA1 in BaP-DNA adduct formation.
Main Methods:
- Developed an immortalized pancreatic ductal epithelial cell line model.
- Utilized luciferase reporter assays, qRT-PCR, Western blotting, immunocytochemistry, and [³H]BaP/ [³²P]-postlabeling assays.
- Employed siRNA to knockdown Aryl hydrocarbon receptor (AhR) and BRCA1.
Main Results:
- The cell model demonstrated intact xenobiotic stress response and AhR signaling activation by BaP.
- No significant BaP-DNA adduct accumulation was observed in AhR-silenced cells.
- BRCA1 knockdown significantly increased BaP-DNA adduct accumulation.
Conclusions:
- The developed in vitro model is feasible for studying pancreatic ductal cell carcinogenesis.
- BRCA1 plays a role in preventing the accumulation of BaP-DNA adducts.
- This model can facilitate future research on dietary mutagens in pancreatic cancer.
