The SOS chromotest study on cisplatin: the genotoxicity evaluation and analytical determination in human urine

Mechanism of action of cis-diamminedichloroplatinum (II) (cis-DDP) was studied using genetically manipulated bacteria Escherichia coli K-12, recombinant PQ 37 (so-called SOS chromotest). Basic characteristics, namely strength of cis-DDP genotoxicity, influence of chloride ions on genotoxicity and dependence of genotoxicity versus time of cis-DDP aquation reaction were tested. The rate constant has been found to be 0.91 +/- 0.03 x 10(-4)s-1. Possibilities of the use of SOS chromotest in the determination of cis-DDP were found. The detection limit has been 0.09 mg/l in water as well as in urine. The amount of cis-DDP was determined in urine of patients after drug treatment. Analysis of clinical samples have been completed without any preseparation.