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Published on: July 28, 2017
Nuclear DNA amounts in angiosperms: targets, trends and tomorrow
1Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, UK.
Annals of Botany
|January 25, 2011
Summary
This study presents the largest compilation of angiosperm DNA C-values, revealing a record rate of genome size data generation. New record holders for smallest and largest genome sizes were identified, expanding the known range significantly.
Area of Science:
- Botany
- Genomics
- Biodiversity Science
Background:
- The C-value, representing DNA content in gametic chromosomes, is crucial for biodiversity and has practical applications.
- Previous compilations of angiosperm C-values have been maintained since 1976, culminating in the Angiosperm DNA C-values Database.
- Significant new data on angiosperm genome size have been published since the last compilation in 2005, necessitating an updated resource.
Purpose of the Study:
- To compile and present the ninth dataset of angiosperm DNA C-values, incorporating recent publications.
- To provide an updated reference for angiosperm genome sizes, now including 6287 species.
- To analyze trends in C-value data generation and representation.
Main Methods:
- Compilation of DNA C-values from 2221 species across 151 original sources, including 1860 new species entries.
- Integration of new data with existing records in the Angiosperm DNA C-values Database.
- Analysis of the expanded dataset to assess data generation rates, genome size ranges, and representation.
Main Results:
- The dataset is the largest to date, with C-values now available for 6287 angiosperm species.
- Angiosperm C-values are being generated at the highest rate observed since the 1950s.
- New record holders for the smallest (Genlisea margaretae, 0.0648 pg) and largest (Paris japonica, 152.23 pg) genome sizes were identified, spanning a 2400-fold range.
- Flow cytometry is emerging as the primary method for genome size estimation, with improved data quality.
- Despite advances in DNA sequencing, it does not yet provide accurate plant genome size estimates.
Conclusions:
- The ninth compilation significantly expands the available data on angiosperm genome sizes.
- The rate of genome size data generation is accelerating, driven by improved methodologies like flow cytometry.
- While representation of genera and regions has improved, familial representation requires further attention.
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