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Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
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Integrative expression system for delivery of antibody fragments by lactobacilli

M Cruz Martín1, Neha Pant, Victor Ladero

  • 1Instituto de Productos Lácteos de Asturias (IPLA, CSIC), 3300 Villaviciosa, Asturias, Spain.

Applied and Environmental Microbiology
|January 25, 2011
PubMed

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...