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Detection of Listeria monocytogenes by using the polymerase chain reaction
M T Bessesen1, Q A Luo, H A Rotbart
1Medical Service, Department of Veterans Affairs Medical Center, Denver, Colorado.
Applied and Environmental Microbiology
|September 1, 1990
Abstract:
A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.