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Poliovirus single-stranded RNA and double-stranded RNA: differential infectivity in enucleate cells

B M Detjen, J Lucas, E Wimmer

    Journal of Virology
    |September 1, 1978
    PubMed

    Insights

    Poliovirus RNA replication was studied in mouse cells lacking a nucleus. Virion RNA replicated, but double-stranded RNA required the nucleus for progeny production, indicating an early nuclear role.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Poliovirus replication is a complex process involving viral RNA.
    • The role of the host cell nucleus in viral RNA replication is not fully understood.

    Purpose of the Study:

    • To investigate the nuclear requirement for poliovirus RNA replication.
    • To differentiate the replication capabilities of poliovirus virion RNA versus double-stranded RNA (replicative form) in enucleated cells.

    Main Methods:

    • Infection of enucleate mouse L cells with poliovirus virion RNA.
    • Infection of enucleate mouse L cells with poliovirus double-stranded RNA (replicative form).
    • Analysis of progeny viral RNA production.

    Main Results:

    • Poliovirus virion RNA successfully replicated in enucleate mouse L cells.
    • Poliovirus replicative form RNA did not produce detectable progeny in enucleate cells.
    • This suggests a nuclear dependence for replication initiated by double-stranded RNA.

    Conclusions:

    • The cell nucleus is essential for the early stages of infection initiated by poliovirus replicative form RNA.
    • Poliovirus virion RNA can replicate independently of the host cell nucleus.
    • These findings highlight distinct nuclear requirements for different forms of poliovirus RNA during infection.

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