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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
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Published on: June 2, 2010

Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters.

Robert M Pasternack1, Bryan Rabin, Jing-Yi Zheng

  • 1Department of Biomedical Engineering, Rutgers University, Piscataway, NJ 08854, USA.

Biomedical Optics Express
|January 25, 2011
PubMed
Summary

This study introduces an optical Fourier filtering method to analyze cell structure changes during apoptosis. The technique detects mitochondrial fragmentation by sensing changes in particle orientation, offering insights into cellular dynamics.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Optical Imaging

Background:

  • Characterizing subcellular morphology is crucial for understanding dynamic cellular functions.
  • Apoptosis involves significant structural changes within cells, particularly in mitochondria and nuclei.
  • Existing methods may lack the sensitivity to detect subtle, early-stage morphological alterations.

Purpose of the Study:

  • To demonstrate a novel optical Fourier filtering method for real-time subcellular morphology analysis.
  • To quantify structural changes in mitochondria and nuclei during early apoptosis (first three hours).
  • To assess the sensitivity of the method to apoptosis-induced changes in particle orientation.

Main Methods:

  • Utilized two-dimensional Gabor elementary functions for Fourier filtering.
  • Tuned filters to specifically detect object size and orientation.
  • Applied the method to analyze mitochondrial and nuclear structures during induced apoptosis.

Main Results:

  • The optical Fourier filtering method detected a decrease in particle orientation in mitochondria, consistent with apoptosis-induced fragmentation.
  • Changes in scattering signals were less pronounced in the nucleus and cytoplasm.
  • Mitochondrial orientation changes were significant, while nuclear and cytoplasmic particle orientations remained relatively stable.

Conclusions:

  • Optical Fourier filtering with Gabor functions is sensitive to early apoptotic changes in mitochondrial morphology.
  • The technique can effectively characterize subcellular structural dynamics, particularly mitochondrial fragmentation.
  • Further studies can explore this method for broader applications in cell biology research.