Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Invasive adenocarcinoma associated with intraductal tubulopapillary neoplasm in young adult mimicking alcoholic pancreatitis and pseudocysts.

Journal of surgical case reports·2026
Same author

Intracellular doppler spectroscopy of live tissue sentinels for a fast in-vitro bacterial infection assay.

Scientific reports·2025
Same author

Evaluating the feasibility and predictive accuracy of biodynamic imaging to platinum-based chemotherapy response in esophageal adenocarcinoma.

Frontiers in oncology·2024
Same author

Immune checkpoint inhibitor-related colitis in patients on immunotherapy for cancer.

American journal of clinical pathology·2024
Same author

Comparative oncology chemosensitivity assay for personalized medicine using low-coherence digital holography of dynamic light scattering from cancer biopsies.

Scientific reports·2024
Same author

Inverse problems in blood flow modeling: A review.

International journal for numerical methods in biomedical engineering·2022

Related Experiment Video

Updated: Jun 5, 2026

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

Mass-transport limitations in spot-based microarrays.

Ming Zhao, Xuefeng Wang, David Nolte

    Biomedical Optics Express
    |January 25, 2011
    PubMed
    Summary

    Analyte transport limits biosensor sensitivity. Molecular interferometric imaging reveals binding variations, enabling accurate kinetics and distinguishing specific from non-specific binding by optimizing flow rates.

    Area of Science:

    • Biophysics
    • Analytical Chemistry
    • Biosensor Technology

    Background:

    • Mass transport of analytes to sensor surfaces is a key limitation in biosensor sensitivity.
    • Analyte depletion causes slow reaction kinetics and uneven binding, hindering accurate measurements.
    • Current methods struggle to quantify binding inhomogeneity and its impact on reaction dynamics.

    Purpose of the Study:

    • To investigate the impact of mass transport on binding homogeneity in microarrays using molecular interferometric imaging (MI2).
    • To develop a method for accurate determination of reaction kinetics by analyzing intra-spot binding inhomogeneity.
    • To identify conditions for homogeneous binding and differentiate specific from non-specific interactions.

    Main Methods:

    • Utilized high-resolution molecular interferometric imaging (MI2), a label-free optical technique, for direct detection of molecular films.

    More Related Videos

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
    13:30

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

    Published on: February 18, 2022

    Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
    08:18

    Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

    Published on: April 24, 2019

    Related Experiment Videos

    Last Updated: Jun 5, 2026

    Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
    10:01

    Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

    Published on: July 30, 2020

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
    13:30

    Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

    Published on: February 18, 2022

    Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
    08:18

    Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes

    Published on: April 24, 2019

  • Studied intra-spot binding distribution across 100-micron protein spots.
  • Combined experimental MI2 data with a 3D finite element model for kinetic analysis.

    • Main Results:

    • Quantified intra-spot binding inhomogeneity, revealing deviations from ideal association kinetics.
    • Identified a critical flow rate, dependent on association rate and spot diameter, for achieving homogeneous binding.
    • Demonstrated that binding inhomogeneity can differentiate high-affinity specific binding from low-affinity non-specific binding.

    Conclusions:

    • Molecular interferometric imaging (MI2) provides a powerful tool to study binding dynamics in biosensors.
    • Optimizing flow rate is crucial for ensuring homogeneous analyte distribution and maximizing sensor performance.
    • Analysis of binding inhomogeneity offers a novel approach for distinguishing specific and non-specific interactions in complex biological samples.