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Towards biochemical filters with a sigmoidal response to pH changes: buffered biocatalytic signal transduction
Marcos Pita1, Vladimir Privman, Mary A Arugula
1Instituto de Catálisis y Petroleoquímica, CSIC, C/Marie Curie 2, 280409 Madrid, Spain.
Abstract:
We realize a biochemical filtering process by introducing a buffer in a biocatalytic signal-transduction logic system based on the function of an enzyme, esterase. The input, ethyl butyrate, is converted into butyric acid--the output signal, which in turn is measured by the drop in the pH value. The developed approach offers a versatile "network element" for increasing the complexity of biochemical information processing systems. Evaluation of an optimal regime for quality filtering is accomplished in the framework of a kinetic rate-equation model.
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