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An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

Development of an oligonucleotide microarray method for Salmonella serotyping.

B Tankouo-Sandjong¹, A Sessitsch, N Stralis-Pavese

¹Austrian Research Centers GmbH, Department of Bioresources, Seibersdorf, Austria.

Microbial Biotechnology

|January 26, 2011

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A new microarray method accurately identifies Salmonella enterica serovars, offering a faster alternative to traditional typing. This advancement aids in rapid epidemiological investigations of foodborne pathogens.

Area of Science:

Microbiology
Molecular Biology
Food Safety

Background:

Accurate Salmonella enterica serovar identification is crucial for epidemiological studies.
Traditional biochemical and serological typing methods are time-consuming.

Purpose of the Study:

To develop a rapid microarray-based method for Salmonella identification.
To differentiate Salmonella species, subspecies, and common serovars.

Main Methods:

Developed a microarray targeting housekeeping (atpD, gyrB) and virulence (fliC, fljB) genes.
Designed 240 probes forming 119 probe units.
Validated the microarray with 57 Salmonella strains and tested on 35 blind samples.

Main Results:

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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

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Last Updated: Jun 5, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

The microarray demonstrated strong discriminatory ability among Salmonella serovars.

Achieved a detection limit of 1 colony forming unit per 25 g of food after 14-hour enrichment.

Successfully identified Salmonella species, subspecies, and 43 S. enterica ssp. enterica serovars.

Conclusions:

The developed microarray is a rapid and effective tool for Salmonella serovar identification.
This method offers a significant improvement over traditional typing for epidemiological surveillance.