Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Allergy testing using degranulation of basophils and flow cytometry.

T A Nilsson1

  • 1Clinical Chemical Laboratory, Central Hospital, Borås, Sweden.

European Journal of Haematology. Supplementum
|January 1, 1990
PubMed
Summary

The Technicon H6000 hematology system effectively detects allergen-induced basophil degranulation in allergy testing. This method, using modified basophil counting, shows strong correlation with traditional skin prick tests and RAST results.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Assay discrepancy in mild haemophilia A.

European journal of haematology. Supplementum·2014
Same journal

On-demand treatment in persons with severe haemophilia.

European journal of haematology. Supplementum·2014
Same journal

Immunosuppressive agents in the treatment of inhibitors in congenital haemophilia A and B--a systematic literature review.

European journal of haematology. Supplementum·2014
Same journal

Perinatal aspects of haemophilia.

European journal of haematology. Supplementum·2014
Same journal

Methods for individualising factor VIII dosing in prophylaxis.

European journal of haematology. Supplementum·2014
Same journal

Outcome measures in haemophilia: a systematic review.

European journal of haematology. Supplementum·2014

Area of Science:

  • Immunology
  • Hematology
  • Allergy Diagnostics

Background:

  • Type I allergy diagnosis relies on allergen-induced reactions.
  • Basophil degranulation is a key indicator of allergic responses.
  • Current diagnostic methods may require further optimization for efficiency.

Purpose of the Study:

  • To evaluate the Technicon H6000 hematology system as a detector for basophil degranulation.
  • To assess the suitability of the H6000 for allergy testing using whole blood samples.
  • To explore modifications for enhanced basophil counting.

Main Methods:

  • Whole blood samples from individuals with type I allergy were used.
  • Diluted allergen extracts were added to blood samples with and without calcium ions.

Related Experiment Videos

  • The Technicon H6000 basophil channel scattergram was employed with a modified counting method.
  • Minimal blood volume (250 microliters) was tested per allergen.

    • Main Results:

    • The Technicon H6000 system demonstrated suitability for allergy testing.
    • Results showed a strong correlation with established skin prick test outcomes.
    • A good correlation was also observed with Radioallergosorbent Test (RAST) results.

    Conclusions:

    • The modified Technicon H6000 system is a viable detector for allergen-induced basophil degranulation.
    • This method offers a potential advancement in whole blood allergy testing.
    • The Technicon H1 system may also be applicable for basophil degranulation studies.