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Optic axis determination by fibre-based polarization-sensitive swept-source optical coherence tomography.

Zenghai Lu1, Deepa K Kasaragod, Stephen J Matcher

  • 1Department of Materials Science and Engineering, The Kroto Research Institute, University of Sheffield, Sheffield, UK. z.lu@sheffield.ac.uk

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|January 26, 2011
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Summary

We developed a novel fiber-based system for 3D optical axis determination in birefringent tissues using polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT). This method accurately measures tissue birefringence and optical axis orientation, validated with equine tendon samples.

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Area of Science:

  • Biomedical Optics
  • Optical Coherence Tomography
  • Biophysics

Background:

  • Birefringent biological tissues possess unique optical properties crucial for understanding their structure and function.
  • Accurate determination of the 3D optical axis in these tissues is essential for various biomedical imaging and diagnostic applications.
  • Existing polarization-sensitive optical coherence tomography (PS-OCT) methods face challenges in precisely measuring optical axis orientation, especially with varying sample arm fiber orientations.

Purpose of the Study:

  • To develop and validate a fiber-based variable-incidence angle (VIA) polarization-sensitive swept-source optical coherence tomography (PS-SS-OCT) system for determining the 3D optical axis of birefringent biological tissues.
  • To investigate a single-plane VIA-PS-OCT approach requiring absolute fast-axis orientation measurement.
  • To compare experimental results with theoretical predictions and assess the robustness of different algorithms for optical axis determination.

Main Methods:

  • Implementation of a fiber-based VIA-PS-SS-OCT system with hardware and software improvements.
  • Acquisition of polarization-sensitive data from equine tendon samples at various illumination angles.
  • Utilizing a quarter-waveplate (QWP) and equine tendon as test targets for validating fast-axis measurements and developing calibration procedures.

Main Results:

  • The VIA method produced polar and azimuthal angles for cut equine tendon that broadly agreed with nominal values (within ~8%).
  • Theoretical and experimental analysis revealed that a previously proposed algorithm for optical axis determination is sensitive to sample arm fiber settings.
  • A new algorithm based on the angle between Stokes vectors on the Poincaré sphere demonstrated reliable performance across all sample arm fiber settings.
  • A calibration procedure was successfully developed and experimentally validated using a QWP to resolve the sign ambiguity in orientation measurements.

Conclusions:

  • The developed fiber-based VIA-PS-SS-OCT system provides a robust method for 3D optical axis determination in birefringent tissues.
  • The Poincaré sphere-based algorithm offers superior performance compared to previous methods, being independent of sample arm fiber orientation.
  • The proposed calibration procedure effectively removes sign ambiguity, enhancing the accuracy and reliability of optical axis measurements.